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제126회 대한화학회 학술발표회 및 총회 Proteomic analysis of environmental stress in correlation with physiological changes in Haliotis discus hannai

등록일
2020년 9월 10일 14시 57분 51초
접수번호
1284
발표코드
ANAL2.O-14 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
화 10시 : 52분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Miseon Jeong, Junghoon Kang, Wonryeon Cho*
Department of Chemistry, Wonkwang University, Korea
With its high market worth and nutrient value for humans, Haliotis discus hannai, also known as the Pacific abalone, is a valuable commercial fishery resource in eastern Asia. Various environmental factors, such as temperature, salinity, oxygen, and CO2 influence its growth and development. Among them, temperature and salinity are the primary physical factors that are affecting the distribution and physiological metabolism of shellfish. These environmentally stressed shellfishes are highly susceptible to pathogens which increase the mortality rate in aquaculture systems. This study aims to determine the physiological changes in H. discus hannai by detecting the protein expression of H. discus hannai which is influenced by changes in water temperature and salinity. Proteins were extracted with RIPA buffer from homogenized hepatopancreas tissue of H. discus hannai. Each supernatant fluid was pooled into experimental and control groups according to the period of exposure to environmental stress. Pooled samples from H. discus hannai were trypsin digested and desalted. Subsequently, the proteins in each group were analyzed with nLC-MS/MS and by protein database searches, and then the effect of environmental stress was also determined with differentially expressed protein (DEP) and gene ontology (GO) enrichment analysis.

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