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초록문의 abstract@kcsnet.or.kr

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  • 03월 07일 12시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Production of human melanocortin-4 receptor TM2 peptide for NMR structural studies

등록일
2007년 2월 13일 11시 50분 44초
접수번호
1036
발표코드
28P141포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
박태준, 최성섭, 김용애
한국외국어대학교 화학과,
The melanocortin receptor family consists of five subtypes (MC1R-MC5R) and belongs to the superfamilly of G protein-coupled receptors (GPCRs) which activate the adenylate cyclase signal transduction pathway. Recently, it has been suggested that normal melanocortin receptor increases energy expenditure and decreases food intake, but genetic disruption of MC4R causes obesity. In fact, 4% of obesity patient have a defect from signal transduction by mutant MC4R/TM2. Therefore, MC4 receptors may be ideal pharmacological targets for treating disorders such as obesity and anorexia. Unfortunately, MC4R is membrane-bound protein that transverse the lipid bilayer of the cell membrane, so it is hard to express and characterize the membrane-bound three-dimensional structure using conventional solution NMR and X-ray crystallography. So far we has been optimized the large scale expression conditions and we are optimizing the purification system to get the pure target membrane protein. Here, we will show the results of expression, purification, and tentative NMR experiments.

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