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Determination of molecular weight distribution and average molecular weights of Oligosaccharides derivatized with 2-aminobenzoic acid by HPLC

등록일
2007년 8월 21일 10시 22분 48초
접수번호
1459
발표코드
32P192포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
김성순, 정원조
인하대학교 화학과,
Size Exclusion Chromatography has been extensively used to monitor molecular weight distribution of polymer samples. Application of SEC for oligosaccharides is widespread, too. However, complete separation of individual oligosaccharides composed of even single monosaccharide by SEC is not possible yet. In this study, a simple method has been developed for determination of molecular weight distribution and average molecular weights. The sample was modified by reductive amination with 4-aminobenzoic acid ethyl ester (ABEE), and ABEE-oligosaccharides were separated in a HPLC system with a common C18 column and a mobile phase of high water content. Baseline separation was observed for ABEE-oligosaccharides. In this system, a larger solute elutes faster than a smaller solute. The merit of this method is the fact that standards are not required since the signal intensity of each ABEE-oligosaccharide is generally proportional to its molar concentration regardless of its molecular weight. The applicability of this method has been examined for a synthetic, a dextran sample, and beer samples.

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