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  • 02월 22일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Specific isoforms of protein kinase G downregulate the transcription of cyclin D1 in NIH3T3

등록일
2008년 2월 13일 17시 17분 16초
접수번호
1151
발표코드
31P47포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
Mohammad M. Khan, 소재원
인하대학교 화학과,
Although protein kinase G (PKG) plays important role in apoptosis induction and cell migration, the isoform specific role of PKG in mediating these functions is still obscure. To elucidate the role of PKG isoforms in transcription control we constructed a series of expression vectors of PKG1α and PKG1β that encode hemagglutinin tagged wild type, constitutively active and dominant negative mutants. Our present study demonstrates that both the constitutively active mutants of PKG 1β downregulate the transcription of cyclin D1 when transiently transfected in NIH3T3 cells, whereas PKG1α mutants show insignificant regulation. We further studied the transcriptional regulators of cyclin D1, such as, c-fos, c-jun, SRE and TRE by using the luciferase reporter assay. Constitutively active mutants of PKG1β showed marked transcriptional downregulation of c-fos in NIH3T3 cells, whereas PKG 1α showed lesser extent. Thus we conclude that the activation of PKG1β in NIH3T3 cells is responsible for the downregulation of cyclin D1.

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