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Analysis of Four Domains Motion of A. aeolicus Argonaute Protein with siRNA using Molecular Dynamics Simulations

등록일
2008년 2월 13일 18시 18분 14초
접수번호
1165
발표코드
31P49포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김성미, 이윤호, 박창인1, 백아영1, 이근우2
경상대학교 응용생명과학부,
1경상대학교 생화학과,
2경상대학교 생화학과 응용생명과학부 EB-NCRC,
Gene silencing mediated by RNA interference (RNAi) depends on short interfering RNAs (siRNAs) and micro RNAs (miRNAs). A siRNA recognizes specific sequences of mRNA through base pairing in RNA-induced silencing complex(RISC). Argonaute proteins are key components of the RISC. They provide both architectural and catalytic functionalities associated with siRNA guide strand recognition and subsequent guide strand-mediated cleavage of complementary mRNAs. Recently crystal structures of archaeal P. furiosus Argonaute(Pf-Ago) and eubacterial A.aeolicus Argonaute(Aa-Ago) were determined with overall architecture which composed of N and PAZ, Mid and PIWI domains. In order to study the key domain motions of Argonaute and the domain interactions with key nucleotide in siRNA, two molecular dynamics simulations were performed over Aa-Ago structure with different substrates including siRNA-mRNA duplex and siRNA single strand. The results have revealed the interaction mode between the siRNA and Aa-Ago protein.

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