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  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Inhibitory effect of ARAC on the inhibition of osteoclastogenesis in Raw 264.7 murine macrophage cells

등록일
2008년 8월 8일 17시 05분 45초
접수번호
0369
발표코드
33P173포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김순남, 이의진1, 김명희2, 민용기3, 김성환4
한국화학연구원, Korea
1한국화학연구원 신약기반기술연구센터, Korea
2충남대학교 생화학과, Korea
3한국화학연구원 생명화학연구단, Korea
4한국화학연구원 화학유전체 연구실, Korea
Osteoclastogenesis is commonly associated with various age-related diseases such as osteoporosis and the receptor activator of nuclear factor-kB (NF-kB) ligand (RANKL) has been shown to play a critical role in osteoclastic bone resorption. With the fact that the development of anti-resorptive agents from natural substances has gained more interest in the treatment of osteoporosis, here, 680 natural compounds were evaluated whether they have a potential to suppress RANKL-induced osteoclastogenesis in RAW264.7 murine macrophage cells. Of these compounds, ARAC was identified as one of compounds inhibiting the RANKL-induced activity of tartrate-resistance acid phosphatase (TRAP). ARAC significantly inhibited the RANKL-induced TRAP activity with IC50, 1.58 ± 0.52 uM and also inhibited the formation of multinucleated osteoclast in a dose-dependent manner. In addition, it was shown to attenuate the RANKL-induced transcript levels of osteoclast specific genes, which have been known to be highly expressed in the process of osteoclastogenesis. Interestingly, ARAC was also shown to inhibit the RANKL-induced activation of mitogen-activated protein kinases. In conclusion, it suggested that ARAC could inhibit the process of osteoclastogenesis through the inhibition of RANKL-induced activation of MAPK signaling pathway.

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