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Stabilization of the InsP6-dependent interaction between CK2α and Nopp140 by Mitoxantrone

등록일
2008년 8월 9일 19시 06분 27초
접수번호
0432
발표코드
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발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
이원규, 이상엽, 조민석, 박찬량1, 유연규
국민대학교 생명나노화학과, Korea
1국민대학교 화학과, Korea
Protein kinase CK2 is a multi-substrate kinase that induces cell proliferation and inhibits apoptosis. Recently, we observed that human nucleolar phosphoprotein p140 (Nopp140) binds to CK2 and represses its catalytic activity. This interaction is prevented by inositol haxakisphosphate (InsP6), and the repressed activity of CK2 by Nopp140 is activated by InsP6. We screened chemical library for chemicals that stabilized the interaction between Nopp140 and CK2α in presence of InsP6. Mitoxantrone (MIX) is found to stabilize the interaction between Nopp140 and CK2α. The binding of MIX to Nopp140 was confirmed by surface plasmon resonance analysis. MIX binds specifically to phosphorylated form of Nopp140 and prevents Nopp140 for binding to CK2α. The activity of CK2 is suppressed by Nopp140, and InsP6. activated the repressed CK2 by preventing the interaction between Nopp140 and CK2. MIX maintains the repressed CK2 activity by Nopp140 by stabilizing the interaction even in the presence of physiological concentrations of InsP6.

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