abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Selective purification of specific proteins in biological sample using surface functionalized superparamagnetic nanoparticles

등록일
2008년 8월 11일 17시 23분 30초
접수번호
0696
발표코드
31P156포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
물리화학
저자 및
공동저자
이연지, 이상원
고려대학교 화학과, Korea
Nano-sized materials have been widely applied to biological system. The Magnetic nanoparticles have been most commonly used for in vivo imaging cell, drug delivery, and cell mechanical studies. Because of the ease of isolation, the separation and purification have become one of the most promising application of magnetic nanoparticles. The surface modification of the nanoparticles makes it possible to isolate the specific species from the complex solution. In our recent experiments, the selective isolation of cysteine-containing peptide from the complex peptide mixture including human serum was achieved by using silica-coated magnetic nanoparticles that were surface-modified with SPDP(N-Succinimidly 3-(2-pyridydithio)-propionate) having reactivity toward free thiol of cysteine. On the basis of this technique, nanoparticles were biofunctionalized with bio-molecule, the tri-peptide containing a cysteine. The target proteins fused with tag protein that recognize bio-molecule as own substrate and bind specifically via enzyme-substrate interaction were isolated without nonspecifically bound proteins from the complex proteome sample. The purified proteins were confirmed by SDS-PAGE and tryptic peptides of those proteins were analyzed by doing LC/MS/MS experiments.

상단으로