abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

The role of hydrophobic motif phosphorylation in the V5 region of Protein Kinase C delta in its activation and subsequent induction of apoptosis in murine fibroblasts

등록일
2008년 8월 11일 17시 32분 43초
접수번호
0707
발표코드
33P199포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
Mrigendra B. Karmacharya, 소재원1
인하대학교 화학과, Nepal
1인하대학교 화학과, Korea
Protein Kinase C delta (PKCδ), one of the important isoforms of PKC, a multi-gene sub-family of serine-threonine kinases consisting of at least 10 isoforms, has been well studied and its role as a pro- as well as anti-apoptotic protein in various cell lines has been established. For its catalytic activity, phosphorylation of serine, threonine and tyrosine residues in various regions are important, especially phosphorylation status of Thr505 in the activation loop, Ser643 in the turn motif, Ser662 in the hydrophobic motif and Tyr311/Tyr322 in the V3 region are crucial. Here, we intended to study the role of the hydrophobic motif, located in the V5 region, in the membrane translocation and activation of PKCδ, and the subsequent induction of apoptosis. For this we generated hydrophobic motif phoshorylation-deficient mutant of PKCδ and compared the catalytic activity of this mutant with wild type PKCδ in murine fibroblast cell line L929. We report that this mutant is catalytically more active and it induced more apoptosis than wild type PKCδ in L929 cells. These results further underscore the importance of the hydrophobic motif in the V5 region of the isoform in its apoptotic activity.

상단으로