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  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Comparison of novel Enzyme-Multiplied Immunoassay Technique (EMIT) and Enzyme-Linked ImmunoSorbent Assay (ELISA) for the determination of residual tetracyclines

등록일
2008년 8월 11일 18시 35분 17초
접수번호
0769
발표코드
32P62포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅳ>
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
전옥금, 전문선, 이인숙
서울여자대학교 화학과, Korea
Tetracyclines (TCs) are a group of broad-spectrum antibiotics which inhibit the bacterial growth by restraining protein synthesis. Due to their ability, they have been widely used for growth promotion and therapeutic purposes in animal husbandry. However, relatively high levels of antibiotic residues in foodstuff can cause a serious threat to human health resulting from increase of drug-resistant strains. To ensure the safety and reliability of food, novel assay designs are still desired. Immunoassays are most powerful tool based on immune reaction that provides high specificity, sensitivity, and simplicity. Immunoassay methods can classify into heterogeneous and homogeneous. EMIT is homogeneous assay format that requires no separation steps, so that it takes few minutes. Also, this method can be easily automated on a dedicated instrument. In case of ELISA as heterogeneous assay, reaction process requires incubation, washing and separation steps, so that slower than EMIT. Accordingly, in this study, we developed the EMIT to detect TC residues and compared EMIT with ELISA.

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