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  • 01월 01일 09시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Development of Advanced Technologies for Comprehensive Proteomics based upon Capillary Reverse-Phase Liquid Chromatography/Tandem Mass Spectrometry

2005년 2월 17일 15시 43분 43초
금14D2심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 14시 : 30분
분석화학 - 최근 질량분석학의 발전
저자 및
고려대학교 화학과,
Mass spectrometry (MS), in combination with separation techniques such as two-dimensional electrophoresis (2DE or 2D-PAGE) or online liquid chromatography, plays an integral role in many aspects of current proteomic researches. These technological advances are enabling proteomics measurements that are increasingly effective, comprehensive and higher throughput. The challenges associated with proteomics measurements include identifying and quantitating large sets of proteins where components of interest may have relative abundances that span more than six orders of magnitude, vary broadly in chemical and physical properties, have transient and low levels of modifications, and are subject to endogenous proteolytic processing. Here we present our efforts to develop high throughput and high informative methodologies for proteomics. They include 1) the development and application of high-resolution, highly sensitive, ultrahigh pressure capillary RPLC systems that operate at the maximum pressure up to 20,000 psig, 2) installation of an electrodynamic ion funnel to improve ion transfer efficiency from high pressure region to low pressure ion guide of electrospray source. We will discuss the utilities of the techniques as viable methods for proteome analyses and present examples of their applications in analyzing samples having different levels of complexity.