119th General Meeting of the KCS

Type Symposium
Area Recent Advances in Analytical Chemistry I: Advanced Materials and Instrument in Analytical Chemistry
Room No. 303호
Time THU 13:30-:
Code ANAL1-1
Subject Flow FFF with Mass Spectrometry for Proteomic and Lipidomic Analysis
Authors 문명희
연세대학교 화학과, Korea
Abstract Flow field-flow fractionation (FlFFF) is a separation method capable of size-sorting particles and biological macromolecules like proteins, DNA, cells, and etc. in an empty channel space without packing materials. During separation of macromolecules by FlFFF, eluting fractions of narrow size range can be collected and utilized for further biological analysis. In this presentation, recent efforts to incorporate mass spectrometry (MS) with FlFFF either by off-line or on-line will be described with applications to proteomic and lipidomic analysis. For the case of off-line combination of FlFFF and MS, subcellular and extracellular species were separated by sizes first and then the collected organelle fractions of different sizes can be further investigated for the proteomic and lipidomic analysis using nanoflow LC-ESI-MS/MS. A miniaturiaed FlFFF can be utilized for the direct hyphenation to MS via electrospray ionization (ESI) or inductively couple plasma (ICP). The former enabled one to have top-down analysis of lipids from lipoproteins without extracting lipids and the latter led us to analyze metals from metalloproteins in blood plasmsa proteome. This presentation introduces recent works on the proteomic/lipidomic analysis of urinary exosomes from prostate cancer patients and on the direct metal analysis of metalloproteins from plasmsa samples with lung cancer.
E-mail mhmoon@yonsei.ac.kr