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|
| Type |
Poster Presentation |
| Area |
고분자화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
POLY.P-2 |
| Subject |
Control of the Hydrophobic and Electrostatic Interaction between Molecular Beacon Aptamer and Conjugated Polyelectrolyte for Detection Range-Tunable ATP Assay |
| Authors |
정지은, 우한영*
고려대학교 이과대학 화학과, Korea
|
| Abstract |
Electrostatic and hydropobic interactions between cationic conjugated polyelectrolytes (CPEs) and molecular beacon aptamer (MBA) makes MBA to have conformation of either open chain or G-quadruplex depending on the presence of a target. MBA is labelled with a fluorophore and a quencher at both termini. In the absence of a target, MBAs have open chain conformation with CPEs, enabling facile FRET from the CPEs to a fluorophore in MBA. By forming a G-quadruplex with a target, strong interaction between the MBA and target ensures the close contact between fluorophore and quencher, resulting in quenched PL signal. Here we report a new strategy of detection range modulation in the CPE-MBA based assay with different stem strength. Modification of ionic density of CPEs suggests an efficient way to fine-control the assay characteristics like detection range and limit of detection. |
| E-mail |
jieunj@korea.ac.kr |
|
119th General Meeting of the KCS