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| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-269 |
| Subject |
Quantitative Analysis and Mapping Neuronal Proteins Using Atomic Force Microscopy
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| Authors |
이동규, 박준원1,*
POSTECH 융합생명공학부, Korea
1POSTECH 화학과, Korea
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| Abstract |
Proteins perform a vast array of functions within living organisms, including catalyzing metabolic reactions, DNA replication, responding to stimuli, and transporting molecules from one location to another. Detection of proteins of low expression level is frequently important, because a certain proteins exist at very low level during the early stages of cell development. In particular, neuronal proteins of the kind play a key role in the regulation of development and synaptic plasticity of neurons.
Conventional methods to analyze proteins include western blotting, ELISA, and immunofluorescence. However, these tools suffer from poor limit of detection, and cannot visualize proteins in high lateral resolution. Therefore, atomic force microscopy (AFM) is a good candidate to overcome the hurdles.
AFM is a powerful tool to get an image of the surface. Moreover, by using the AFM it can show information about the distribution of the biomolecule. Using the mapping capability and imaging mode of AFM, it is possible to obtain an image of a single neuron and map the distribution of a specific protein in a nanometeric resolution.
In the congress, we present our effort to demonstrate such capability of AFM.
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| E-mail |
lee2012@postech.ac.kr |
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119th General Meeting of the KCS