119th General Meeting of the KCS

Type Poster Presentation
Area 생명화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code BIO.P-270
Subject Surface Structure of Amyloid beta Oligomers and Fibrils Analyzed by Atomic Force Microscopy
Authors 윤지현, 박준원*
POSTECH 화학과, Korea
Abstract Atomic force microscopy (AFM) can image topography of biomolecules with the sub-nanometer resolution. Furthermore, since AFM imaging can be performed in an aqueous solution, it has been applied under physiological condition. AFM is also used to measure the interaction strength of biomolecules with the pico-Newton resolution. Based on the AFM imaging mode and force measurement, we can image the distribution of interaction site on sample with high spatial resolution and sensitivity. Here, we investigated amyloid beta (Aβ) oligomers and fibrils that are believed to play important roles in Alzheimer’s disease (AD) by using AFM force mapping mode. AD is one of the most prevalent severe degenerative neuronal diseases afflicting our elderly population. AD is characterized by synapse and neuron loss in the brain, the accumulation of senile plaques which are protein deposits, and neurofibrillary tangles. The Aβ is believed to play important causal roles in AD. Although many investigators studied such neurotoxic Aβ, the molecular structure of them remains unclear. We employed AFM to elucidate the surface structure of Aβ. Observed specific interaction between Aβ and both C/N-terminal specific antibody suggests a surface structure model in which both N-terminus and C-terminus are exposed at the surface.
E-mail yoonjihyun@postech.ac.kr