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Type |
Poster Presentation |
Area |
생명화학 |
Room No. |
포스터발표장 |
Time |
4월 21일 (금요일) 13:00~14:30 |
Code |
BIO.P-272 |
Subject |
Supersensitive Analysis of MicroRNA Using Metal-Enhanced Fluorescence Nanoparticles [동우화인켐㈜ 대학(원)생 선정 포스터상]
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Authors |
박수현, 남좌민1, 박준원* POSTECH 화학과, Korea 1서울대학교 화학부, Korea |
Abstract |
MicroRNAs(miRNAs) are known to be involved in protein transcription, physiological mechanisms such as development, differentiation, cell proliferation and apoptosis. They are also known to be associated with many diseases such as cancer, Alzheimer’s disease, myocardial disease. miRNA is 22mer shorter than general RNA, thus miRNA is amplified by PCR. In particular, the role of miRNAs in cancer has been extensively studied. miRNA analysis can be useful for early diagnosis or treatment of diseases.
Currently, Polymerase Chain Reaction (PCR) is a standard method to detect miRNA. However, it causes error in amplification process several times and analysis sensitivity is relatively low. In order to overcome the limitations of the conventional technology, I will improve the sensitivity more than 100 times by the fusion of fluorescence enhanced nanoparticles and microarray.
Here we suggest new approaches to supersensitive analysis of miRNA using metal-enhanced fluorescence (MEF) nanoparticles and microarray. To detect miRNA, we use S9.6 antibody that is DNA/RNA hybrid-specific mouse monoclonal antibody. This approach is expected to perform parallel analysis with detection sensitivity increased several hundred times more than the conventional methods.
Reference
1. Z. Hu, et al. Nucl. Acid Res. 2006, 34, e52
2. B. J. Hong, et al. Nucl. Acid Res. 2005, 33, 12
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E-mail |
parksoo@postech.ac.kr |
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