119th General Meeting of the KCS

Type Symposium
Area Current Trends in Organic Chemistry Ⅱ: Molecular Recognition
Room No. 302호
Time FRI 09:25-:
Code ORGN2-2
Subject Detection of Heavy Metal Ions and polysaccharides in Aqueous Solution with Fluorescence probes Using Aggregation-Induced Emission process
Authors 이건형
인하대학교 화학과, Korea
Abstract Smart fluorescent probes of which the detection of specific molecules can be controlled are interesting. Fluorescent probes were synthesized by conjugating aggregation-induced emission (AIE) fluorophores with peptide receptors for metal ions and heparins and its contaminant oversulfated chondroitin sulfate (OSCS). The selective detection of dipeptidy probe (1) for specific metal ion in aqueous solutions was controlled by the buffering agents without the change of pH. In phosphate buffered solution, 1 exhibited a selective Off-On response to a soft metal, Hg2+ by 100-fold enhancement of the emission. 1 showed a selective Off-On response to a hard metal, Al3+ in Tris and Hexamine buffered aqueous solution. The detection limits were lower than the maximum allowable level of the metal ions in drinking water by EPA. The fluorescent tripeptidyl probe using an AIE fluorophore exhibited a sensitive turn-on response to heparin, an anticoagulant polysaccharide, in aqueous solution. The probe showed a highly selective response to heparin among biological competitors in human serum samples. Unlike other chemical probes for heparin, the peptidyl probe recognized heparin and then formed nano-sized aggregates, which was potent resistant against heparinase. The dual role of the fluorescent probe for the detection of heparin and inhibition for heparinase made it possible for the selective and sensitive detection of contaminated OSCS in heaprin. This research helps to understand how buffering agents control the selective detection of fluorescent probes using an AIE process for the metal ions in aqueous solutions and the biomedical application of the aggregation process could go far beyond simply sensing.
E-mail leekh@inha.ac.kr