|
|
| Type |
Poster Presentation |
| Area |
의약화학 |
| Room No. |
포스터발표장 |
| Time |
4월 20일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-419 |
| Subject |
Validated high-performance liquid chromatography method for the inhibitor of cancer cell invasion in rat plasma and its application to pharmacokinetic study
|
| Authors |
최현경*, 김정수
중원대학교 의약화학과, Korea
|
| Abstract |
In this study, a reliable method for the quantitation of (E)-N-benzyl-6-(2-(3, 4-dihydroxy benzylidene)hydrazinyl)-N-methylpyridine-3-sulfonamide (JW-55) in rat plasma was developed and validated using high-performance liquid chromatography (HPLC). Plasma samples were deproteinized; sildenafil was used as an internal standard. Chromatographic separation was achieved using a reversed-phase (C18) column. The mobile phase, 0.02 M ammonium acetate buffer:acetonitrile (48:52, v/v), was run at a flow rate of 1.0 mL/min at room temperature, and the column eluent was monitored using an ultraviolet detector at 280 nm. The retention times of JW-55 and sildenafil were approximately 5.9 and 7.7 min, respectively. The detection limit of JW-55 in rat plasma was 0.03 mg/mL. Pharmacokinetic parameters of JW-55 were evaluated after intravenous and oral administration of JW-55 (10 mg/kg) in rats. After oral administration, the F value was approximately 73.7%.
|
| E-mail |
hkchoi45@hanmail.net |
|
119th General Meeting of the KCS