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| Type |
Poster Presentation |
| Area |
분석화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
ANAL.P-173 |
| Subject |
Direct Screening of H7N9 Virus DNA based on Spectral Imaging without Target Amplification |
| Authors |
이승아, 강성호*
경희대학교 응용화학과, Korea
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| Abstract |
Total internal reflection scattering (TIRS) along with a transmission grating (TG) was effectively utilized for quantitative DAN screening without amplification process. Gold nanopad was used as a substrate for analyzing hybridization of single-stranded DNA (ssDNA)-TIRS nanotag (silver nanoparticles). The TG separated the scattering signals of the nanotag from the substrate and displaced the optical path of the scattered signals to avoid errors caused by several acquisitions, especially for studying plasmonic metal tagged samples (i.e., influenza A; H7N9). This nanobiosensor based on sandwich hybridization quantified the target virus DNA until 74 zM (LOD) without interference of the gold nanopad substrate by using TG. The development is a significant advance in the development of a PCR-free biosensor to greatly reduce the time of sample pretreatment for quantitative screening of influenza A (H7N9) virus. |
| E-mail |
moon1131@gmail.com |
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119th General Meeting of the KCS