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| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-277 |
| Subject |
Combining suppression of stemness with lineage-specific induction leads to conversion of pluripotent cells into functional neurons |
| Authors |
HALDER DEBASISH, 신인재1,*
연세대학교 화학, Korea
1연세대학교 화학과, Korea
|
| Abstract |
Sox2 is a key player in the maintenance of pluripotency and stemness, and thus inhibition of its function would abrogate stemness of pluripotent cells and induce differentiation into several types of cells. Herein we describe a strategy, which relies on a combination of Sox2 inhibition with lineage-specific induction, for promoting efficient and selective differentiation of pluripotent P19 cells into neurons. When P19 cells transduced with Skp protein, an inhibitor of Sox2, are incubated with a neurogenesis inducer, the cells are selectively converted into neurons which generate depolarization-induced sodium currents and action potentials. This finding indicates that the differentiated neurons are electrophysiologically active. Signaling pathway studies lead us to conclude that a combination of Skp with the neurogenesis inducer enhances neurogenesis in P19 cells by activating Wnt and Notch pathways. The present differentiation protocol could be valuable to selectively generate functionally active neurons from pluripotent cells. |
| E-mail |
deba_genetic@yahoo.com |
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119th General Meeting of the KCS