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| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-278 |
| Subject |
Synthetic ion transporters were based on calixpyrrole that perturb cellular chloride ion homeostasis induce caspase mediated apoptosis |
| Authors |
박성현, 신인재*
연세대학교 화학과, Korea
|
| Abstract |
Synthetic ion transporters have received great attention as therapeutic agents because of their potential to perturb cellular ion homeostasis. However, a direct correlation between a change in cellular ion concentration and cytotoxicity has not been established for synthetic ion carriers. Herein we show that two pyridine diamide-strapped calix[4]pyrroles induce coupled chloride anion and sodium cation transport in both liposomal models and cells, and promote cell death by increasing intracellular chloride and sodium ion concentrations. Removing either ion from the extracellular media or blocking natural sodium channels with amiloride prevents this effect. The results of cell experiments indicate that the ion transporters promote the sodium chloride influx, which results in an increased concentration of reactive oxygen species, release of cytochrome c from the mitochondria and apoptosis via caspase activation. However, they do not activate the caspase-independent apoptotic pathway associated with the apoptosis-inducing factor. The present study suggests that artificial ion transporters represent an attractive approach for modulating cellular processes that are normally controlled tightly by homeostasis. |
| E-mail |
heaven_bell@yonsei.ac.kr |
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119th General Meeting of the KCS