119th General Meeting of the KCS

Type Poster Presentation
Area 생명화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code BIO.P-280
Subject A Sirtuin 1 selective inhibitor induces neuronal differentiation of pluripotent P19 cells
Authors 이창희, 신인재*
연세대학교 화학과, Korea
Abstract Sirtuin 1 (SIRT1), a histone deacetylases, is known to play important roles in neuronal differentiation of pluripotent / multipotent stem cells by regulating expression of neuron-associated genes. Several inhibitors of SIRT1 have been exploited for use in diverse biological functions, however, most of these inhibitors also known to function on other Sirtuin family proteins. Herein, we employed a potent and selective small molecule inhibitor of SIRT1 to test whether inhibition of SIRT1 activities can lead to the differentiation of P19 cells into neurons. In this effort, P19 cells were treated for 9 days with the selective inhibitor of SIRT1, EX-527, and then performed immunocytochemical, western blot and RT-PCR analyses. The results showed that EX-527 promotes neuronal differentiation of P19 cells without differentiation into astrocytes and cardiac cells. The findings indicate that the inhibitor induces selective neurogenesis of P19 cells. In addition, the differentiated neurons from P19 cells treated with EX-527 generated voltage-dependent sodium currents and depolarization-induced action potentials. These findings clearly indicate that the neuron-like cells have electrophysiological properties. The present study suggests that SIRT1 selective inhibitors could have the potential as chemical inducers to differentiate pluripotent cells into functional neurons.
E-mail leech12311@yonsei.ac.kr