|
Type |
Poster Presentation |
Area |
물리화학 |
Room No. |
포스터발표장 |
Time |
4월 20일 (목요일) 11:00~12:30 |
Code |
PHYS.P-31 |
Subject |
Dark Matter miRNA detection using surface-enhanced Raman scattering based on well-designed nanostructure |
Authors |
권혁상 한국표준과학연구원(KRISS) 미래융합기술본부/나노바이오측정센터, Korea |
Abstract |
MicroRNAs (miRNAs) are small non-coding RNA molecules that have functions in silencing. Recently, the unraveled expression of miRNAs is recognizing as disease states relating with cancer, kidney disease, obesity and nervous system. Herein, we aim to develop ultrasensitive and multiplexed detection platform of miRNAs existing in dark matter of cancer patient no showing therapeutic efficacy for anticancer drug based on surface-enhanced Raman scattering or spectroscopy (SERS). First, we synthesized complementary DNAs with one-end Raman marker and the other-end thiol group, which were self-assembled for the formation of self-assembled monolayer on the SERS substrate with a well-designed nanostructure. Then, we flowed target miRNAs to make DNA:RNA double strands. In this time, Raman signal is “ON” state. To confirm the formation of DNA:RNA duplex through sequence specific hybridization, we finally treated duplex-specific nuclease (DSN) because it has a strong preference for cleaving double stranded oligonucleotides. Once sequence specific hybridization occurs on the SERS substrate, DSN would recognize duplex efficiently followed by degradation of probe DNA, which causes releasing SERS signaling molecule from the surface (“OFF” state). By changing target miRNA with different Raman probes, it will be applicable to the diagnosis of cancer patient based on the dark matter detection under the multiplexed SERS detection. |
E-mail |
h.kwon@kriss.re.kr |
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