119th General Meeting of the KCS

Type Symposium
Area Biomacromolecules and Protein Engineering
Room No. 403호
Time FRI 10:10-:
Code BIO2-3
Subject FACS-based enzyme engineering for the enhanced production of biomolecules
Authors 정기준
KAIST 생명화학공학과, Korea
Abstract Currently, proteins have indeed found significant applications in various bioprocesses but the full realization of their potential has been limited. To acquire the desired properties, proteins need to be engineered and, for this purpose, directed evolution techniques has been the most powerful tool. Directed evolution consists of an iterative two-step protocol, initially generating molecular diversity by random mutagenesis, then identifying library members with improvements in desired phenotype by screening or selection, and in many cases, the success of directed evolution highly rely on the employed screening strategy. Our research is also focused on the development of efficient strategy for high throughput screening and particularly, we have developed FACS-based high throughput screening in bacterial hosts. In this presentation, I will introduce new screening strategy for the engineering of industrial enzymes towards enhanced production of biomolecules.
E-mail kjjeong@kaist.ac.kr