119th General Meeting of the KCS

Type Poster Presentation
Area 분석화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code ANAL.P-177
Subject Detection and optimization of sample preparation of ricin by immunomagnetic separation and liquid chromatography-tandem mass spectrometry
Authors 권지현, 이용한*
국방과학연구소 5본부 3부, Korea
Abstract Ricin is a toxic protein derived from castor beans and consist of a cytotoxic A chain and a galactose-binding B chain covalently linked by a disulfide bond, which can inhibit protein synthesis and cause cell death by binding the surface of cell. Due to its high toxicity, ricin has been listed as both chemical and biological warfare agents in Chemical Weapon Convention (CWC). The direct analysis of ricin by mass soectrimetry is not possible because ricin is a ~62kDa protein with a high level of structural heterogeneity. To overcome this limitation is to work on enzymatic digest of the ricin. Our studies were focused on the optimization of sample preparation of ricin. We indroduced the immunomagnetic separation (IMS) method to isolate the ricin from the matrix, allowing the extracted ricin to be further digested and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Through this study, the optimized sample preparation was applied to first biotoxin sample analysis proficiency test from the organization for the prohibition chemical weapons (OPCW).
E-mail jhkwon8@add.re.kr