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| Type |
Poster Presentation |
| Area |
물리화학 |
| Room No. |
포스터발표장 |
| Time |
4월 20일 (목요일) 11:00~12:30 |
| Code |
PHYS.P-38 |
| Subject |
Binding interaction of propyl gallate with bovine and human serum albumins by fluorescence spectroscopy |
| Authors |
이성준, 조한국*, 정병서*
인천대학교 화학과, Korea
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| Abstract |
The interaction of the antioxidant, propyl gallate (PG) with bovine and human serum albumins in phosphate buffer has been studied by fluorescence spectroscopy. The intrinsic fluorescence of serum albumins was monitored as a function of PG concentrations. Fluorescence quenching assays were used to characterize the binding affinity of PG. The fluorescence quenching constants were determined based on the Stern―Volmer and modified Stern―Volmer equations. In addition, the binding constant and the number of binding sites between serum albumins and PG were determined by the Scatchard-type analysis. The site marker displacement experiments were performed to identify the binding site of serum albumins using warfarin and ibuprofen as site markers. |
| E-mail |
leesjch@naver.com |
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119th General Meeting of the KCS