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| Type |
Poster Presentation |
| Area |
의약화학 |
| Room No. |
포스터발표장 |
| Time |
4월 20일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-428 |
| Subject |
Comparison of ISE and ICP-MS to Determine Iodine in Urine |
| Authors |
조효정
녹십자의료재단 대사체연구팀, Korea |
| Abstract |
Iodine, an essential element for thyroid function, is necessary for normal growth, development, and functioning of the brain and body. Iodine-deficiency disorder (IDD) is a well documented global health problem, affecting more than a billion people worldwide. Consequences of IDD include goiter, cretinism, intellectual impairment, brain damage, mental retardation, stillbirth, spontaneous abortions, miscarriages, congenital deformities, and increased perinatal mortality.
Urinary iodine values directly reflect dietary iodine intake, urinary iodine analysis is the recommended and most common method for biochemically assessing the iodine status of a population. This method achieves rapid and accurate quantification of iodine content in urine.
We evaluated the analytical performance of inductively coupled plasma-mass spectrometry (ICP-MS) and Ion-selective electrodes (ISE) to determine urinary iodine concentration. And the 2 methods for iodine were compared for the 24hr urine samples(n=105).
Material & Method
• ICP-MS method
20 uL of the internal standard solution (Indium, 100ug/L) and 2 mL of dilution solution (0.05% triton X-100, 0.2% NH4OH, 2% 1-butanol, 0.05% ehtylendiaminetetraacetic acid diammonium salt hydrate in d.w) were added into 100 uL of urine then vortex mixed for 30 sec. And injected into the 7900x ICP-MS system(Agilent, USA).
- Iodine (127)
- Indium (IS) (115)
• ISE method
40 uL of the ISA solution and 1.8 mL d.w were added into 200 uL of urine then vortex mixed for 30 sec. And injected into the A214 ISE iodide electrode. (Thermo, USA).
Conclusion
The difference % of two methods is within a ±20%. But ISE method is lower precision than ICP-MS and value have increased over time. It seems that iodine in urine is not entirely free iodide, and free iodine becomes increasingly with time.
ISE electrode is influenced by pH, acids, matrix etc. So the application of ISE is often necessary quality control to check the accuracy.
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| E-mail |
yaong6@gclabs.co.kr |
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119th General Meeting of the KCS