|
Type |
Symposium |
Area |
Recent Advances in Analytical Chemistry Ⅱ: Microfluidics for Novel Diagnostics |
Room No. |
303호 |
Time |
FRI 15:30-: |
Code |
ANAL2-3 |
Subject |
Wash-free magnetic immunoassay of hazardous materials using a fully automated SERS-based microdroplet platform |
Authors |
주재범 한양대학교 생명나노공학과, Korea |
Abstract |
We report the rapid and sensitive immunoassay of hazardous materials using a surface-enhanced Raman scattering (SERS)-based microdroplet sensor. This sensor enables a convenient immunoassay of a specific marker without any washing process. Herein, magnetic immunocomplexes can be easily isolated by the droplet splitting into two smaller parts. One is the droplet including magnetic immunocomplexes and the other is the supernatant droplet including unbound SERS nano tags. In this system, droplet generation, transport, immunoreactions, isolation of immunocomplexes, droplet fission are sequentially performed to achieve a wash-free immunoassay in an automatic manner. SERS signals for unbound SERS nano tags in separated droplets were measured and analyzed for the quantitative analysis of a target marker. SERS signals for approximately 200 droplets/min (3.0 Hz) were measured and averaged for the quantitative evaluation of a target marker. In this system, a tiny volume of sample is needed since all the process can be automatically carried out in a specially designed microdroplet channel. We anticipate that this fully integrated SERS-based microdroplet device opens new insights in the development of a facile assay platform for various hazardous materials. |
E-mail |
jbchoo@hanyang.ac.kr |
|