119th General Meeting of the KCS

Type Poster Presentation
Area 분석화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code ANAL.P-182
Subject Simultaneous analysis and time-concentration profiles of soman and VX adducts to butyrylcholinesterase and albumin in rhesus monkey plasma by liquid chromatography-tandem mass spectrometry
Authors 이진영, 권지현, 이용한*
국방과학연구소 5본부 3부, Korea
Abstract A sensitive method for the purification and determination of two protein adducts, organophosphorus (OP)-BChE and OP-albumin adducts in a single sample of the use simultaneously in the sample preparation step has been developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Firstly, we used immunomagnetic separation (IMS) method to isolate the OP-BChE adducts and the HiTrapTM Blue affinity column was followed by used to isolate and purify OP-albumin adducts from the rhesus monkey plasma that was exposed to nerve agents. Also, we studied the time-concentration profiles of two biomarkers, OP-nonapeptides and OP-tyrosines derived from OP-BChE and OP-albumin adducts up to 8 weeks and knew that OP-tyrosines are more favorable biomarkers than OP-nonapeptides due to their long life time. We expect that this intergrated approach will also be used to quantify other types of OP-BChE adducts and OP-albumin adducts in humen plasma, thus serving as a possible general assay for biomarkers of exposure to nerve agents.
E-mail marlintime@add.re.kr