|
Type |
Poster Presentation |
Area |
분석화학 |
Room No. |
포스터발표장 |
Time |
4월 21일 (금요일) 13:00~14:30 |
Code |
ANAL.P-182 |
Subject |
Simultaneous analysis and time-concentration profiles of soman and VX adducts to butyrylcholinesterase and albumin in rhesus monkey plasma by liquid chromatography-tandem mass spectrometry |
Authors |
이진영, 권지현, 이용한* 국방과학연구소 5본부 3부, Korea |
Abstract |
A sensitive method for the purification and determination of two protein adducts, organophosphorus (OP)-BChE and OP-albumin adducts in a single sample of the use simultaneously in the sample preparation step has been developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Firstly, we used immunomagnetic separation (IMS) method to isolate the OP-BChE adducts and the HiTrapTM Blue affinity column was followed by used to isolate and purify OP-albumin adducts from the rhesus monkey plasma that was exposed to nerve agents. Also, we studied the time-concentration profiles of two biomarkers, OP-nonapeptides and OP-tyrosines derived from OP-BChE and OP-albumin adducts up to 8 weeks and knew that OP-tyrosines are more favorable biomarkers than OP-nonapeptides due to their long life time. We expect that this intergrated approach will also be used to quantify other types of OP-BChE adducts and OP-albumin adducts in humen plasma, thus serving as a possible general assay for biomarkers of exposure to nerve agents. |
E-mail |
marlintime@add.re.kr |
|