119th General Meeting of the KCS

Type Poster Presentation
Area 무기화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code INOR.P-82
Subject Diiridium (III) complexes as luminescent probes and sensors for G-quadruplex DNA and endoplasmic reticulum imaging
Authors 김진흥*, TIKUM FLORENCE ANJONG1, 임윤진2
이화여자대학교 화학·나노과학과, Korea
1이화여자대학교 화학나노과학과 무기화학전공, Korea
2이화여자대학교 화학나노과학과, Korea
Abstract G-quadruplex is a four stranded structure, derived from a guanine (G) rich DNA sequence. Though G-rich sequences have been demonstrated to form g-quadruplex structure invitro, the existence of g-quadruplex structure invivo is still controversial. Thus detecting g-quadruplex structure has great significance for cell proliferation, cancer research and drug development. Also, imaging of small organelles in the cell such as ER is very important, in understanding the structure and function of these organelles. Herein, two new dinuclear iridium (Ir) complexes bridged by a conjugate aromatic tppz ligand, were prepared. The DNA binding properties of both complexes where studied using single, double stranded and g-quadruplex DNA. Interestingly, the Ir complex demonstrated selectivity for g-quadruplex DNA over other control DNA which was observed by an enhancement in the phosphorescent spectra of 1. Inorder to understand their possible interaction with lipid-rich organelles in live cells, the phosphorescent properties of both complexes were studied using liposomes which showed an increased in phosphorescent intensity upon the addition of liposomes with complex showing a greater enhancement. The complex could be used as probes in live cells. Complexes were intensely stained in HeLa cervical cancer cells. Excitation with a l = 488 nm laser showed red emission localized in the cytosol of cells. Cellular studies showed that 1 and 2 were located inside HeLa cells, but neither complex passed through the nuclear membrane. However, complexes possessed superior photostability, indicating their role as good luminescent agents for imaging and tracking the endoplasmic reticulum in live cells. Overall, the complex exhibited better selectivity for G-quadruplexes, liposomes, and ER staining.
E-mail jinheung@ewha.ac.kr