|
|
| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-287 |
| Subject |
Mechanistic study of O-ureidoserine racemase (DcsC) [우수포스터상] |
| Authors |
안영찬
University of Alberta Chemistry, Canada |
| Abstract |
O-ureidoserine racemase (DcsC) is a PLP-independent racemase involved in the biosynthesis of important antibiotic D-cycloserine.1 Our goal is to elucidate the mechanism of this unusual epimerization by means of crystallization of the enzyme with a known optically pure inhibitor.2 Alternatively mechanistic information can be achieved by crystallization of the racemic inhibitor with site-specifically mutated DcsC variants. This approach has been shown successful in proving the proposed epimerization mechanism for the highly homologous diaminopimelate epimerase (DapF, 40% homology).3 With a crystal structure in hand we will be able to visualize the 3D geometry of the active site revealing how DcsC is able to significantly decrease the pka of the substrate thus enabling the proton abstraction under physiological conditions .
1 Uda et al. Antimicrob. Agents Chemother, 2013, 57, 2603-2612
2 Dietrich et al., Org. Biomol. Chem. 2012, 10, 2248-2254.
3 B. Pillai et al., Proc. Natl. Acad. Sci. 2006, 103, 8668-8673.
|
| E-mail |
yeongcha@ualberta.ca |
|
119th General Meeting of the KCS