119th General Meeting of the KCS

Type Poster Presentation
Area 생명화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code BIO.P-288
Subject Development of inner ear-targeted drug delivery system with phospholipid nanoemulsion
Authors 손지환, 구희범1,*
가톨릭대학교 의생명건강과학과, Korea
1가톨릭대학교 의과대학/의생명과학교실, Korea
Abstract The purpose of this study is to develop an inner ear-targeted drug delivery system with phospholipid nanoemulsion. To optimize particles, we varied charges or attached polyethylene glycol (PEG) for safety and efficiency. Anionic, neutral, cationic particles and cationic-PEG particles was fabricated. PEG was attached to induce long circulation inside perilymph by avoiding non-specific binding with proteins. The sizes of candidate particles were about 200 nm. First, their cellular uptake was observed in HEI-OC1 cell. In FACS analysis, cationic particles showed most intense fluorescence, and cation-PEG, neutral, anionic particles were followed. However, in artificial mucosa exam, fluorescence intensity inside mucosa and HEI-OC1 cells below the mucosa was highest in case of cationic-PEG particles and neutral particles. The overall results demonstrate that cationic particles showed highest cellular uptake during in vitro exams similar to the previous studies, but to permeate RWM and deliver a drug to hair cells after circulating perilymph, attaching PEG or using neutral particles seems favorable.
E-mail jayson@catholic.ac.kr