119th General Meeting of the KCS

Type Poster Presentation
Area 분석화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code ANAL.P-203
Subject Plasmon-enhanced photoluminescence immunoassay for Influenza virus sensing by using gold nanoparticle decorated carbon nanotube
Authors 오상진, 김정효1, 이재범2,*
부산대학교 인지메카트로닉스공학과, Korea
1부산대학교 인지메카트로닉스 공학과, Korea
2부산대학교 광메카트로닉스공학과, Korea
Abstract

A plasmon-assisted fluoro-immunoassay (PAFI) was developed for the detection of the influenza virus by using Au nanoparticle (Au NP)-decorated carbon nanotubes (AuCNTs) that were synthesized using phytochemical composites at room temperature in deionized water. Specific antibodies (Abs) against the influenza virus were conjugated onto the surface of AuCNTs and cadmium telluride quantum dots (QDs), which had a photoluminescence intensity that varied as a function of virus concentration and a detection limit of 0.1 pg/mL for all three types of influenza viruses examined. The clinically isolated influenza viruses (A/Yokohama/110/2009 (H3N2)) were detected in the range of 50–10,000 PFU/mL, with a detection limit of 50 PFU/mL. From a series of proof-of-concept and clinical experiments, the developed PAFI biosensing system provided robust signal production and enhancement, as well as an excellent selectivity and sensitivity for influenza viruses. This nanoparticle-based technique could be potentially developed as an efficient detection platform for the influenza virus.

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