119th General Meeting of the KCS

Type Poster Presentation
Area 분석화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code ANAL.P-211
Subject Shotgun proteomics-based global proteome analysis of colon cancer cell line from two-and three-dimensional cell culture
Authors 김영은, 강덕진1,*
한국표준과학연구원(KRISS) 삶의질측정본부 바이오임상표준센터, Korea
1한국표준과학연구원(KRISS) 삶의질측정표준본부, Korea
Abstract Two-dimensional (2D) cell culture system is well established strategy to perform in vitro cell-based studies regarding to unearthing biological functions of proteins and metabolites in a cell, as well as, in particular, drug(s) screening for cancer cells. Even though 2D cell culture has been considered as a fundamental way to cell-based in vitro studies, it has been, unfortunately, reported that the results concerning both cytotoxicity and activity of drug on 2D cell culture are not fully matched with that of in vivo tissues. Very recently, various strategies for three-dimensional (3D) cell culture have been developed by means of matrix-based or matrix-free platforms, so as to mimic in vivo environment of cells. Based on recent study on 3D cell culture, 3D-cultured cells in morphology, gene/protein expression and drug sensitivity are quite different from that of 2D-cultured cells. In this study, we analyzed the cellular proteome from 2D-cultured colon cancer cell line SW480 and 3D cultured one, thereby profiling the proteome difference according to 2D/3D culture conditions. To do so, we performed a comprehensive proteomic analysis of 2D- and 3D-cultured cells using 2D-nanoLC-ESI-MS/MS experiments. Furthermore, the quantitative assessment of cellular proteome from 2D-/3D-cultured cells also was carried out by using isobaric tags for relative and absolute quantitation (iTRAQ) labeling. Based on our experiment, we found that a number of proteins identified in common from both of 2D-/3D-cultured SW480 are shown to be significantly different between two different culture platforms. From doing this proteomic analysis of 3D-culutred cell line, it allows to be boosted up in discovery of drug that is effective against diverse cancer.
E-mail yekim@kriss.re.kr