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Type |
Poster Presentation |
Area |
의약화학 |
Room No. |
포스터발표장 |
Time |
4월 20일 (목요일) 11:00~12:30 |
Code |
MEDI.P-433 |
Subject |
The Simultaneous Analysis of Essential Trace Elements in Human Breast Milk by ICP-MS |
Authors |
조효정 녹십자의료재단 대사체연구팀, Korea |
Abstract |
Recently, analytical methods have some light on the nutritional importance of essential elements in human breast milk. Trace elements analyses in human breast milk have been previously reported using various methologies. ICP-MS has become one of the most sensitive, accurate and reliable analytical techniques for determination of trace elements. Previous studies have reported involving microwave with acid digestion at various temperatures. But this methods are time-consuming and expensive, highly losses by volatilization, contamination, hazardous use of mineral acids.
The main objectives this study were to determination trace elements(Zn, Cu, Mn) and stability in human breast milk.
Material & Method
• Study sample
A certified reference material for human breast milk is currently not available, and pooled human breast milk samples as well as standard reference materials for formula milk powder such as NIST 1549a to validate the methods.
All samples were frozen at -20℃ until analysis. And samples were thawed at room temperature.
• ICP-MS method
80 uL of the internal standard solution (Indium, 100ug/L) and 1.6 mL of dilution solution (2% HNO3 and 0.01% triton X-100 in d.w) were added into 160 uL of sample. It vortex mixed for 30 sec and centrifuge 13,200 rpm, 4℃. Injected into the 7900x ICP-MS system(Agilent, USA).
- Manganese (55), Copper(63), Zinc(66)
- Indium (Internal standard) (115)
Conclusion
The method is precise with intra-day and inter-day repeatability values of <7 and <6% in pooled breast milk. The intra-day and inter-day repeatability values for certified reference material NIST 1549 for elements were <4 and <6 %.
Our method has shown high repeatability and sensitivity, as well as linearity within a wide range of values. This method is applicable for analysis of other essential and toxic elements in human breast milk.
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E-mail |
yaong6@gclabs.co.kr |
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