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| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-292 |
| Subject |
Biological effect of modified AuNPs depending on the surface charge and ligand structure |
| Authors |
고종연, 이의연, 권영은*
동국대학교 의생명공학과, Korea
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| Abstract |
AuNPs are widely utilized for numerous biomedical applications as they exhibit superior biocompatibility and marginal toxicity. AuNPs can be modified to obtain various physic-chemical properties and it is important to understand how the physico-chemical properties of AuNPs affect their biological interactions. Among many variables, we here focused on the effect of surface chemistry, i.e. charge and structure of ligands. For a systematic approach, we prepared mono-dispersed AuNPs carrying various functional groups based on a sequential ligand exchange method. The prepared AuNPs had surface charges ranging from -36.1±1.4 mV to +37.1±0.5 mV. Various biological assays were performed to measure the effects of each AuNPs on cell viability as well as on various cellular functions. Uptake efficiency of AuNPs by mammalian cells and the subsequent changes of cell morphologies and cytoskeletal structures were also monitored. These studies showed that the cytotoxicity of anionic and neutral AuNPs were insignificant, whereas cationic AuNPs showed mixed effects from non-toxic to severely toxic, depending on the structure of ligands. While all the cationic AuNPs tested were more effectively internalized by mammalian cells compared with anionic or neutral AuNPs, only the cytotoxic MUAM-AuNP showed inhibitory effects on the DNA replication and on the formation of actin filament. The results showed that not the charge of the AuNPs but the structure of the ligand was an important factor determining the biological effects of AuNPs. This work also proposed that properly designed cationic AuNPs could make a good candidate for low toxicity gene-delivery materials. |
| E-mail |
diiiiiiiiib73@gmail.com |
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119th General Meeting of the KCS