119th General Meeting of the KCS

Type Symposium
Area Current Trends in Organic Chemistry Ⅱ: Molecular Recognition
Room No. 302호
Time FRI 10:00-:
Code ORGN2-3
Subject Ratiometric Two-Photon Probes for Imaging pH Values in Live Samples
Authors 김환명
아주대학교 화학과/에너지시스템학과, Korea
Abstract During the past decade, two-photon microscopy (TPM) has emerged as a powerful tool to imaging studies of biology and medicine. TPM uses two near-infrared (NIR) photons as the excitation source and offers several advantages, including inherent sectioning capability, low photo-damage, longer observation time, and greater tissue penetration depth. To facilitate the use of TPM in biomedical applications, there is a strong need to develop a variety of molecular TP probes for direct, quantitative measurement of target analyte in cell, tissue, and organism level. Recently, we have developed small molecule and emission ratiometric TP probes possessing a significant two-photon brightness, a marked emission color change, easy loading, high photostability, and low cytotoxicity. Intracellular pH varies greatly among individual subcellular compartments. Many metabolic pathways are regulated by pH values. To extend our understanding of the roles of pH in biology and pathology, it is crucial to monitor subcellular pH values and their fluctuation at the cell, tissue, and organism level. In this talk, the design, evaluation, and applications of selected TP probes for pH will be presented.
E-mail kimhm@ajou.ac.kr