|
|
| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Material Chemists |
| Room No. |
402호 |
| Time |
THU 10:20-: |
| Code |
MAT.O-9 |
| Subject |
Injectable hydrogels derived from alginate-alendronate calcium complexes for bone regeneration |
| Authors |
김한샘, 신원상1,*
단국대학교 나노바이오의과학과, Korea
1단국대학교 나노바이오의과학, Korea
|
| Abstract |
Modification of sodium alginate was conducted by the functionalization of alginate with Alendronate and calcium complex formation using Ca(OAc)2 finally to get alginate-alendronate calcium complexes (Alg-Alen/Ca) containing calcium and phosphate ions. The obtained alginate derivatives including Alginate-Alendronate (Alg-Alen) and Alginate-Alendronate calcium complex (Alg-Alen/Ca) were characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy for 1H nuclei, high resolution inductively coupled plasma optical emission spectroscopy, and thermogravimetric analysis. The new injectable hydrogels (Alg-Alen/Ca/Alg) were simply prepared by mixing Alg-AlenCa solution with NaAlg solution in different volume ratios (40:60, 50:50, 60:40) without additional calcium salt supply externally. The gelation was completed within 5-15min indicating a high potential of hydrogel delivery by injection to in vivo. SEM analysis of the prepared injectable Alg-Alen/Ca/Alg hydrogels showed highly porous morphology with interconnected pores of width in the range of 180-630μm. In vitro 3D cell study using mesenchymal stem cell showed that the injectable hydrogels exhibited comparable properties to the cell culture plate in terms of cytotoxicity and differentiation of MSCs. The developed injectable hydrogels showed a high degree of biocompatibility and significantly higher ALP gene and ARS expressions, suggesting the potential application in bone tissue engineering and regenerative medicine. |
| E-mail |
drywind010@dankook.ac.kr |
|
119th General Meeting of the KCS