119th General Meeting of the KCS

Type Symposium
Area Recent Advances in Analytical Chemistry Ⅱ: Microfluidics for Novel Diagnostics
Room No. 303호
Time FRI 14:30-:
Code ANAL2-1
Subject Applications of Digital Microfluidic Platform in Clinical Diagnostics
Authors 최기환
한국표준과학연구원(KRISS) 유기분석표준센터, Korea
Abstract Digital microfluidics (DMF) is a fluid handling technique in which discrete droplets are manipulated on the surface of an array of electrodes. The open nature of DMF systems enables the use of heterogeneous samples, such as dried blood spots and hydrogels. In this talk, I will present two recent projects using solid samples on a DMF device. First, I will describe the work implementing magnetic particle-based immunoassays in DMF. To increase the assay throughput, a new platform capable of performing complete immunoassays with minimal manual intervention was developed. This instrument comprises three core components: a Pogo pin interface for digital microfluidic control, an integrated photomultiplier tube for chemiluminescent detection, and an adjustable magnet for particle separation. Relative to macroscale methods, this DMF approach reduced reagent volumes and analysis time. Second, I will present an analytical technique to quantify the multiplexed steroid from tiny amount of tissue samples. Digital microfluidic actuation of methanol allowed to extract the analytes from milligram-sized tissue samples. The extractant was further processed in a DMF device with porous polymer monoliths prepared by photopolymerization of a C12 casting solution. The solution was injected and analyzed by HPLC-MS/MS for quantification of four hormones: estradiol, testosterone, androstenedione progesterone. This represents the first method capable of quantification of these analytes in core needle biopsy (CNB) samples, and suggests a future in which regular CNB testing is applied to a personalized medicine approach to diagnosis and treatment of hormone-sensitive cancer.
E-mail kihwan.choi@kriss.re.kr