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| Type |
Symposium |
| Area |
[IBS 심포지엄] Genome Editing and the CRISPR/Cas Revolution (※IBS 유전체 교정 연구단 공동개최) |
| Room No. |
302호 |
| Time |
WED 16:20-: |
| Code |
KCS4-4 |
| Subject |
Highly efficient RNA-guided base editing in mouse embryos |
| Authors |
김경미, 김진수1,*
기초과학연구원 유전체교정연구단, Korea
1서울대학교 화학부, Korea
|
| Abstract |
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44%-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations. |
| E-mail |
rudal0912@naver.com |
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119th General Meeting of the KCS