|
|
| Type |
Poster Presentation |
| Area |
의약화학 |
| Room No. |
포스터발표장 |
| Time |
4월 20일 (목요일) 11:00~12:30 |
| Code |
MEDI.P-442 |
| Subject |
Anti-oxidative and anti-cholinesterase compounds from Galla Rhois |
| Authors |
권희수*, 정규선1, 이봉호2,*, 최병욱*
한밭대학교 화학생명공학과, Korea
1한밭대학교 생명공학과, Korea
2한밭대학교 응용화학생명공학부, Korea
|
| Abstract |
In our continuing search for anti-oxidative, anti-cholinesterase and anti-tyrosinase compounds from oriental medicinal plants, Galla Rhois was examined. Here we report the progress on the purification, biological activities and structural determination of the two isolated compounds. From the 100% methanol extract of Galla Rhois, solvent partition fractions were prepared by using n-hexane(14-GRM-H), chloroform(14-GRM-C), butanol(14-GRM-B) and water(14-GRM-W). Among them 14-GRM-B showed strong anti-oxidative, BuChE* inhibitory and anti-tyrosinase activities. The 14-GRM-B of Galla Rhois showed 84% DPPH radical scavenging activity, 81% BuChE inhibitory activity and 62% tyrosinase inhibitory activity at 100 ppm. Using a series of ODS open column chromatographic purification steps, two compounds, 14-GRM-B-333 and 14-GRM-B-335 could be purified. At 100 ppm, they showed anti-oxidative activity as 93.9, 94.5%, anti-tyrosinase inhibitory activity as 70.9, 89.6%, and AChE** inhibitory activity as 50.3, 64.7%, respectively. The structures of 14-GRM-B-333 and 14-GRM-B-335 were determined by 1H and 13C NMR and MS analysis. The structures of 14-GRM-B-333 and 14-GRM-B-335 were turned out to be methyl gallate and PGG(pentagalloylglucose), respectively.
* BuChE : Butyrylcholinesterase, ** AChE : Acetylcholinesterase |
| E-mail |
gmltn5512@naver.com |
|
119th General Meeting of the KCS