119th General Meeting of the KCS

Type Poster Presentation
Area 생명화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code BIO.P-300
Subject Paper chip-based simple, rapid, colorimetric detection of kanamycin using ssDNA aptamer
Authors 하나름, 정인필, 김아루, 김상헌, 윤문영*
한양대학교 화학과, Korea
Abstract Kanamycin is an one of the most important subclasses of aminoglycoside antibiotics which has been widely used to treat diverse infections. However, residual kanamycin can cause various side effects in humans such as loss of hearing, kidney toxicity. The risk of antibiotic residues in meat and milk is well known and focuses on intensive regulatory processes. Thus it is necessary to develop highly sensitive diagnostic kit for residual antibiotics. In this study, we developed the label-free AuNPs-based colorimetric aptasensors on paper chip. The 13 nm AuNPs were synthesized by citrate reduction of HAuCl4 and paper chips were designed and printed on LFNC 80 membrane using a wax-based printer. The citrate-coated AuNPs were modified with the 39 mer kanamycin binding aptamer for 15 min at RT with mild shaking. Then different concentration of kanamycin was added, and after the addition of high concentration of NaCl into the mixed solution. The visual results were monitored by naked eye and measured the delta RGB value. Kanamycin was detected until very low pM range within 30 min. In conclusion, the proposed system could accurately detect kanamycin and this results demonstrate the designed system could be applied for rapid detection of kanamycin in real samples.
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