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| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-302 |
| Subject |
Development of a ssDNA aptamer to detect the Nonylphenol ethoxylate with Graphene oxide |
| Authors |
김아루, 하나름, 정인필, 김상헌, 윤문영*
한양대학교 화학과, Korea
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| Abstract |
An endocrine disruptor is a major public health problem to people throughout the world. In the human body, the endocrine disruptors functionalize same as a sexual hormone with inducing a precocious puberty, gynecomastia, etc. Nonylphenol, one of the endocrine receptor, has attracted attention due to its prevalence in the environment and its ability to act with estrogen-like activity and it arises from the environmental degradation of Nonylphenol ethoxylates (NPEs), which is used to make detergents and it can also act as endocrine disruptor. So, NPEs should be detected easily and fast to prevent taking from laundry or dish and damaging caused by it and its metabolite.
Herein, we developed a novel single strand DNA (ssDNA) aptamer for detection of NPEs. For this process, graphene oxide (GO) is used to gather unbound ssDNA with the counter target and GO-bound ssDNA is eluted with the target. We found aptamers and characterized with AuNP. Synthesized aptamers can bind to NPEs with high binding affinity. The aptamers have Kd value from tens of to hundreds of nanomolar (nM) level. Specificity of the aptamers also tested. One of them can distinguish specifically NPEs from the other chemicals We expect the aptamer can be a useful tool on a sensitive detection system towards NPEs for people’s health. |
| E-mail |
kimr2122@gmail.com |
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119th General Meeting of the KCS