|
|
| Type |
Poster Presentation |
| Area |
생명화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
BIO.P-310 |
| Subject |
Hybrid immobilization with magnetic iron oxide nanoparticles of an Esterase (EaEST) from Exiguobacterium antarcticum |
| Authors |
김부영, 유완기1, Wang Ying, 김두헌2,*
숙명여자대학교 화학과, Korea
1성균관대학교 의과대학, Korea
2숙명여자대학교 이과대학 화학과, Korea
|
| Abstract |
A novel immobilization of EaEST was performed by co-aggregation with magnetite nanoparticles (MNPs) and glutaraldehyde as cross-linking reagent. The hybrid aggregated EaEST with MNPs was analyzed by scanning electron microscopy and confocal laser scanning microscopy. In this study, the hybrid magnetic cross-linked EaEST (HM-EaEST-CLEAs) retained its activity by 70%, relative activity to soluble Ha28, over 18 cycles of reuse. HM-EaEST-CLEAs was analyzed by various biochemical methods for confirming the efficiency. Compared with free enzyme and standard CLEAs, the HM-EaEST-CLEAs exhibited high thermostability. And high stability also revealed in harsh states such as various pH levels and solvents. In addition, we carried out kinetics assay which was revealed the improvement of the immobilized efficiency. These characteristics make it promising as a biocatalyst for industrial processes. |
| E-mail |
tito2010@naver.com |
|
119th General Meeting of the KCS