|
Type |
Poster Presentation |
Area |
생명화학 |
Room No. |
포스터발표장 |
Time |
4월 21일 (금요일) 13:00~14:30 |
Code |
BIO.P-311 |
Subject |
A near-infrared fluorescent sensor with a self-immolative linker for the quantitative detection and imaging of hydrogen sulfide in vivo |
Authors |
박철순, 하태환1, 이경관2, 이순혁3, 이창수4,* 전남대학교 대학원 고분자공학과, Korea 1한국생명공학연구원 바이오모니터링연구센터, Korea 2한국생명공학연구원(KRIBB) 위해요소감지BNT연구단, Korea 3과학기술연합대학원대학교(UST) 나노바이오공학과, Korea 4한국생명공학연구원 바이오나노연구센터, Korea |
Abstract |
Hydrogen sulfide is a critical biological messenger, but few biologically compatible methods are available for its detection in vivo. Here, we describe the design and synthesis of a novel azide-functionalized near-infrared probe, NIR-Az, for a hydrogen sulfide assay in which a self-immolative linker is incorporated between the azide moiety and phenolic dihydroxanthene fluorophore from a cyanine dye. A large “turn-on” near-infrared fluorescence signal results from the reduction of the azide group of the fluorogenic moiety to an amine, in which the self-immolative linker also enhances the accessibility of NIR-Az to hydrogen sulfide. NIR-Az can select hydrogen sulfide from among 16 analytes, including cysteine, glutathione, and homocysteine. By exploiting the superior properties of NIR-Az, such as its good biocompatibility and rapid cell internalization, we successfully demonstrated its usefulness in monitoring both the concentrationand time-dependent variations of hydrogen sulfide in living cells and animals (detection limit less than 0.26 μM), thereby providing a powerful approach for probing hydrogen sulfide chemistry in biological systems. |
E-mail |
log71944@gmail.com |
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