119th General Meeting of the KCS

Type Poster Presentation
Area 생명화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code BIO.P-311
Subject A near-infrared fluorescent sensor with a self-immolative linker for the quantitative detection and imaging of hydrogen sulfide in vivo
Authors 박철순, 하태환1, 이경관2, 이순혁3, 이창수4,*
전남대학교 대학원 고분자공학과, Korea
1한국생명공학연구원 바이오모니터링연구센터, Korea
2한국생명공학연구원(KRIBB) 위해요소감지BNT연구단, Korea
3과학기술연합대학원대학교(UST) 나노바이오공학과, Korea
4한국생명공학연구원 바이오나노연구센터, Korea
Abstract Hydrogen sulfide is a critical biological messenger, but few biologically compatible methods are available for its detection in vivo. Here, we describe the design and synthesis of a novel azide-functionalized near-infrared probe, NIR-Az, for a hydrogen sulfide assay in which a self-immolative linker is incorporated between the azide moiety and phenolic dihydroxanthene fluorophore from a cyanine dye. A large “turn-on” near-infrared fluorescence signal results from the reduction of the azide group of the fluorogenic moiety to an amine, in which the self-immolative linker also enhances the accessibility of NIR-Az to hydrogen sulfide. NIR-Az can select hydrogen sulfide from among 16 analytes, including cysteine, glutathione, and homocysteine. By exploiting the superior properties of NIR-Az, such as its good biocompatibility and rapid cell internalization, we successfully demonstrated its usefulness in monitoring both the concentrationand time-dependent variations of hydrogen sulfide in living cells and animals (detection limit less than 0.26 μM), thereby providing a powerful approach for probing hydrogen sulfide chemistry in biological systems.
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