119th General Meeting of the KCS

Type Poster Presentation
Area 생명화학
Room No. 포스터발표장
Time 4월 21일 (금요일) 13:00~14:30
Code BIO.P-312
Subject A sandwich immunoassay for detection of Mycobacterium tuberculosis secretory antigen using recombiant antibodies
Authors 이승우, 김종길, 박태정*
중앙대학교 화학과, Korea
Abstract Mycobacterium tuberculosis (M.tuberculosis) is a serious infectious pathogen causing tuberculosis (TB). Because it affects the lungs and spread easily through the air, TB has become a global threat leading people to deaths especially in developing countries. To prevent TB spreading and also improve patient care, rapid and accurate diagnosis method of TB is required for its early diagnosis. In this study, one of the secretory antigen of M.tb was chosen as a target for early TB diagnosis. Three different single-chain antibody fragments (scFv) against target were expressed in Escherichia coli. These antibodies were analyzed for its binding affinities with the target through SPR analysis. With these recombinant antibodies, a rapid and sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for TB detection was developed. The antibodies fused to alkaline phosphatase (AP) was constructed, expressed and validated by the AP enzyme activities. Moreover, binding properties of AP-fused antibody with target was confirmed through colorimetric analysis and proved its efficiency as a detection antibody. With this, the sandwich ELISA was performed with wide-range concentration of target and the results showed high sensitivity and selectivity towards target proving its great potential as a TB diagnosing tool.
E-mail swish0507@naver.com