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|
| Type |
Poster Presentation |
| Area |
전기화학 |
| Room No. |
포스터발표장 |
| Time |
4월 21일 (금요일) 13:00~14:30 |
| Code |
ELEC.P-536 |
| Subject |
Impedimetric cholera toxin biosensor based on β-galactose derivative functionalized gold surface |
| Authors |
정대호, 이원용*
연세대학교 화학과, Korea
|
| Abstract |
Cholera toxin, an oligomeric complex secreted by Vibrio cholera, which causes dehydration and severe diarrhea due to the binding of B subunits to GM1 ganglioside on the surface of mucous cells in the small intestine. More specifically, cholera toxin is a hexameric protein complex consist of five identical B subunits and an active single A subunit. The five identical B subunits are specifically bind to pentasaccharide ganglioside receptor. It is estimated that 3 to 5 million are infected and approximately 100,000 are killed by cholera per year worldwide. Due to its acuteness, cholera patients require immediate diagnosis and round the clock care. Several analytical methods were developed for CT detection such as fluorescence bioassay and immunoassay. These are based on antibody and GM1 moiety, but they have some limitations such as lack of stability, time-consuming and expensiveness. In the present study, a simple, rapid, label-free and effective cholera toxin biosensor has been developed by using electrochemical impedance spectroscopy (EIS) between modified gold electrode functionalized with synthesized monosaccharide β-galactose and cholera toxin. The present study demonstrates a great analytical performance of β-galactose modified Au electrode in terms of sensitivity and low detection limit as 800 fM which is much lower than the previous study. |
| E-mail |
jeongteddh@yonsei.ac.kr |
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119th General Meeting of the KCS