|
|
| Type |
Symposium |
| Area |
[English Session - IBS 심포지엄] New Directions in Polymer Science (※IBS 첨단연성물질 연구단 공동개최) |
| Room No. |
405호 |
| Time |
FRI 09:00-: |
| Code |
POLY2-1 |
| Subject |
Studying the Dynamics of Complex Coacervates with Super-Resolved Microscopy |
| Authors |
John King*, Anisha Shakya1
기초과학연구원 (IBS) 첨단연성물질연구단, United States
1기초과학연구원 첨단연성물질연구단, Nepal
|
| Abstract |
Solutions comprised of oppositely charged polyelectrolytes assemble into liquid-liquid phase separated coacervates. The practical function, often exploited by living cells, is to isolate, concentrate and protect components of the cell without the use of membranes. To carry out these vital functions, coacervates must remain highly dynamic and avoid aggregation and entanglement of the components. Here, we study the dynamics of in vitro polymer-ATP and polymer-ATP-DNA coacervates using a combination of super-resolved fluorescence microscopy and fluorescence correlation spectroscopy (FCS). This experimental approach allows the diffusion of the comprising species to be studied as a function of length scale, ranging from 200 nm (diffraction limited) to 60 nm. We find a strong dependence of the local diffusion on the measurement length scale, which is not consistent with simple Brownian diffusion. |
| E-mail |
jtking.ibs@gmail.com |
|
119th General Meeting of the KCS