119th General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Material Chemists
Room No. 402호
Time THU 10:50-:
Code MAT.O-12
Subject Multiplexed detection strategy using optokinetically encoded nanoparticles on lipid bilayer
Authors 김선기, 남좌민*
서울대학교 화학부, Korea
Abstract Multiplexed real-time analysis on multiple interacting molecules and particles is needed to obtain valuable information in a complex medium, often found in chemical and biological systems. In particular, multiplexed profiling of microRNA (miRNA) in a reliable, quantitative manner is of great demand for the use of miRNA in cell biology, biosensing and clinical diagnostic applications. Here, we report a multiplexed miRNA detection strategy using optokinetically (OK) coded nanoprobes (NPs) that show distinct optical signals and dynamic behaviors on a supported lipid bilayer (SLB) (OK-NLB assay).[1] Metal NPs with three distinct dark-field light scattering signals [red (R), green (G) and blue (B)] and three different target miRNA half-complements were tethered to two dimensionally fluid SLB with mobile (M) or immobile (I) state. In situ single-particle monitoring and normalized RGB analysis of the optokinetically combinatorial assemblies between three M-NPs and three I-NPs with dark-field microscopy (DFM) allow for differentiating and quantifying 9 different miRNA targets in one sample. The OK-NP-based assay enables simultaneous detection of multiple miRNA targets in a highly quantitative, specific manner within 1 hour, and can be potentially used for diagnosis of different cancer types.
E-mail sungikim@snu.ac.kr