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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists Ⅰ |
| Room No. |
303호 |
| Time |
THU 09:30-: |
| Code |
ANAL1.O-8 |
| Subject |
Immunoreaction-based dual-wavelength capillary electrophoresis for simultaneous detection of thyroid hormones |
| Authors |
우나인, 강성호1,*
경희대학교 일반대학원 화학과, Korea
1경희대학교 응용화학과, Korea
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| Abstract |
Immunoreaction-based capillary electrophoresis (CE) with a laser-induced fluorescence (LIF) detector was developed for the fast and highly sensitive detection of thyroid hormones. Thyroid hormones have been reported to influence various diseases such as hypothyroid, hyperthyroid, thyroidectomy, and thyroiditis. Therefore, measurements of T4, T3, and TSH are useful for the clinical evaluation of thyroid function. In this study, immunoreaction and biotin-streptavidin interaction were investigated for highly sensitive detection of thyroid hormones as a function of migration time and separation efficiency. Various factors such as the effective length, separation voltage, and pH of running buffer were also examined in order to determine the optimal CE conditions. All three standard thyroid hormones were analyzed within 3.2 min in 25 μM Na2B4O7-NaOH buffer (pH 9.3) without losing resolution under optimum conditions, which were 1,000-100,000 times more sensitive than other previous detection methods. In addition, the CE with LIF detection method also successfully applied to the analysis of real human blood samples. Consequently, the immunoreaction-based CE method with LIF detection is an effective, rapid analysis technique for highly sensitive detection of thyroid hormones related to thyroid gland disease. |
| E-mail |
936050@naver.com |
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119th General Meeting of the KCS